发明名称 |
Peptide analysis using a solid support |
摘要 |
The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
|
申请公布号 |
US2004171070(A1) |
申请公布日期 |
2004.09.02 |
申请号 |
US20040478910 |
申请日期 |
2004.04.28 |
申请人 |
BHIKHABHAI RAMAGAURI;LIMINGA MARIA;MALOISEL JEAN-LUC;PALMGREN RONNIE;KEOUGH THOMAS W;KEOUGH AMY M;YOUNGQUIST ROBERT S;VAUGHN HAROLD L;YELM KEN E |
发明人 |
BHIKHABHAI RAMAGAURI;LIMINGA MARIA;MALOISEL JEAN-LUC;PALMGREN RONNIE;KEOUGH THOMAS W;KEOUGH AMY M;YOUNGQUIST ROBERT S;VAUGHN HAROLD L;YELM KEN E |
分类号 |
C07K1/12;G01N33/543;G01N33/68;(IPC1-7):G01N33/53 |
主分类号 |
C07K1/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|