| 摘要 |
A method for producing a clone of an immortalised human B memory lymphocyte, comprising the step of transforming human B memory lymphocytes using Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocyte capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of: <SL> <LI>i) selecting and isolating a human memory B lymphocyte subpopulation, <LI>ii) transforming the subpopulation with Epstein Barr virus (EBV) in the presence of a polyclonal B cell activator, <LI>iii) screening the culture supernatant for antigen specificity and <LI>iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity. </SL> |
