发明名称 |
Vectors encoding HCN channels and MiRP1 |
摘要 |
This invention provides for a chamber and system designed for use in assaying drug effects on heart rate. The chamber consists of a series of wells, each 3 mm by 3 mm in inner diameter. Cardiac myocytes disaggregated from neonatal animals are plated onto the bottom of each well and grown under standard tissue culture conditions. The chamber holds from 24-96 such wells. When drugs are to be assayed, the cells in each well are loaded with a calcium sensitive dye and the beating rate in each is monitored with a photodiode. Drug is added in graded concentrations to each well, and equilibrated and effects on rate are observed. This construct permits use of a cell based bioassay for the study of drugs or agents that may alter cardiac rate. This invention can be used in high throughput screening of drugs to evaluate/predict their effects on cardiac rate and rhythm. Further provided for by this invention is a vector which comprises a compound which encodes an ion channel.
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申请公布号 |
US6783979(B2) |
申请公布日期 |
2004.08.31 |
申请号 |
US20010875392 |
申请日期 |
2001.06.06 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK |
发明人 |
ROSEN MICHAEL R.;ROBINSON RICHARD B.;COHEN IRA S.;YU HAN-GANG |
分类号 |
A61K38/17;A61K48/00;G01N33/50;(IPC1-7):C12N15/00;C12N15/64;C12N15/86;A01N63/00 |
主分类号 |
A61K38/17 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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