发明名称 |
Methods for quantitative analysis of nucleic acid amplification reaction |
摘要 |
A method for determining a threshold cycle number or time value in a nucleic acid amplification reaction comprising the steps of amplifying a nucleic acid sequence in a reaction mixture; at a plurality of different times during the amplification reaction, measuring at least one signal whose intensity is related to the quantity of the nucleic acid sequence in the reaction mixture; and storing signal values defining a growth curve for the nucleic acid sequence. A derivative of the growth curve is determined and the threshold cycle number or time value is calculated as a cycle number or time value corresponding to a characteristic (e.g., maximum, minimum, or zero-crossing) of the derivative.
|
申请公布号 |
US6783934(B1) |
申请公布日期 |
2004.08.31 |
申请号 |
US20000562195 |
申请日期 |
2000.05.01 |
申请人 |
CEPHEID, INC. |
发明人 |
MCMILLAN WILLIAM A.;CHRISTEL LEE A.;BORKHOLDER DAVID A.;YOUNG STEVEN J. |
分类号 |
B01L7/00;C07H21/02;C07H21/04;C12M1/00;C12M1/34;C12N9/98;C12P19/34;C12Q1/00;C12Q1/68;G01N21/27;G01N21/64;G01N33/48;G01N33/50;G01N35/00;G06F19/00;(IPC1-7):C12Q1/68 |
主分类号 |
B01L7/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|