Oligonucleotide linkers comprising a variable cohesive portion and method for the praparation of polynucleotide libraries by using said linkers

摘要

A linker or population of linkers comprising an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is A, C, G, T or U, or their derivatives, and n is an integer equal to or higher than 1. A linker-polynucleotide or a population of linker-polynucleotides comprising said linker or population of linkers and a target first strand polynucleotide bound to said linker. A method of preparing said linker or population of linkers and a method of preparing a linker-polynucleotide using said linker or population of linkers. Provided is a linker instead of G tailing, which can be utilized in a method of preparing a cDNA library, and a method of preparing a cDNA library using said linker.