METHOD OF ELIMINATING PHOSPHATE GROUP OF PEPTIDE AND METHOD OF ANALYZING PEPTIDE

摘要

It is intended to provide a method of eliminating a phosphate group of a peptide by a chemical treatment without resort to any enzymes and a method of efficiently analyzing a peptide by using the above method. Namely, a method of eliminating a phosphate group of a peptide by using a reagent containing at lease one member selected from the group consisting of hydrogen fluoride, hydrofluoric acid and a hydrogen fluoride-containing compound, and a method of analyzing a peptide by using this method. As the hydrogen fluoride-containing compound, it is preferable to employ hydrogen fluoride-pyridine. The elimination of a phosphate group of a peptide is carried out by controlling the total amount of hydrogen fluoride in hydrogen fluoride, hydrofluoric acid and the hydrogen fluoride-containing compound contained in the reagent to 10 to 100% by weight at a reaction temperature of -10 to 50°C. The analysis of a peptide is carried out preferably by mass spectroscopy with the use of MALDI-TOFMS.