POLYMERASE-BASED PROTOCOLS FOR GENERATING CHIMERIC AND COMBINATORIAL CHIMERIC OLIGONUCLEOTIDES

摘要

The present invention relates to methods of generating chimeric oligonucleotides without the need for subcloning. The methods of the invention are polymerase-based, and may optionally be adapted for use with reagents available in commercially available mutagenesis kits such as Stratagene's QCM kits, to generate chimeric oligonucleotides in a quick, efficient and cost-effective manner. Major applications of this method include vaccine production, directed evolution and other areas that benefit from the development of diversity.