Low temperature expression cDNAs encoding fructan synthesizing enzymes and method of isolating the same

摘要

A wheat-derived sucrose:fructan 6-fructosyltransferase cDNA is provided which is characterized in that said cDNA has a nucleotide sequence corresponding to an amino acid sequence listed as SEQ.ID. No. 1 in FIG. 1. A wheat-derived invertase cDNA is provided which is characterized in that said cDNA has a nucleotide sequence corresponding to an amino acid sequence listed as SEQ.ID. No. 2 in FIG. 2. A method is provided for isolating a wheat-derived sucrose:fructan 6-fructosyltransferase cDNA having a nucleotide sequence corresponding to an amino acid sequence listed as SEQ.ID. No.1 in FIG. 1 and a wheat-derived invertase cDNA having a nucleotide sequence corresponding to an amino acid sequence listed as SEQ.ID. No. 2 in FIG. 2.