METHOD FOR CLEAVING AND DEGLYCOSYLATING ANTIBODIES TO PROMOTE LIGAND BINDING
摘要
The present invention details methods to detect glycoproteins in samples to identify a disease state. In particular, an improved sandwich assay is described, using both an antibody and an array of lectins. Methods for increasing the efficiency of interaction of an antibody with a ligand by cleaving the antibody to remove the carbohydrate moiety, and orienting the cleaved fragment on a matrix to enhance recognition of a ligand are also described. Methods of differentiating pregnancy-derived and disease derived samples are also described.
申请公布号
WO03087821(A3)
申请公布日期
2004.08.19
申请号
WO2003US10673
申请日期
2003.04.07
申请人
UNIVERSITY OF GEORGIA RESEARCH FOUNDATION;KELLY, LISA S., PH.D.;PUETT, DAVID, J.;PIERCE, MICHAEL, J.
发明人
KELLY, LISA S., PH.D.;PUETT, DAVID, J.;PIERCE, MICHAEL, J.