METHOD FOR ISOLATING GONADAL PRIMORDIAL GERM CELLS AND METHOD FOR PREPARING GERMLINE CHIMERIC AVES

摘要

PURPOSE: A method for isolating gonadal primordial germ cells and a method for preparing germline chimeric aves are provided, thereby improving purity and isolation yield of gonadal primordial germ cells, and improving preparation efficiency of germline chimeric aves. CONSTITUTION: The method for isolating gonadal primordial germ cells comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; and (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex. The method for preparing germline chimeric aves comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex; (d) introducing the isolated gonadal primordial germ cells into the embryo of a recipient egg; and (e) culturing and hatching the egg.