Compositions and methods for identifying thymidylate synthase modulators

摘要

The present invention provides compositions and methods for screening of compounds that decrease the activity of thymidylate synthase either directly or indirectly. The composition of the invention comprises a DNA construct comprising from the 5' to the 3' direction, a TBE cassette comprising one or more copies, in forward or reverse orientation, of TBE1 and TBE2, and a reporter gene operably linked to a promoter and optionally a selectable marker. The method of the invention comprises growing cells comprising the construct of the invention in the presence or absence of a test compound such that a test compound which decreases the activity of