Quantitative determination method for heparan sulfate and diagnostic method using the same

摘要

The present invention relates to a quantitative determination method for heparan sulfate contained in body fluid specimens, which method is useful for identifying various chronic diseases. The present invention also provides means for judging the condition of diabetic nephropathy by determination of heparan sulfate contained in a urine specimen by use of the quantitative determination method of the present invention. The present invention further provides means for diagnosing hepatic diseases and rheumatoid arthritis by determination of heparan sulfate contained in a blood specimen by use of the quantitative determination method of the present invention. The inventors of the present invention have found that heparan sulfate changes quantitatively and/or qualitatively in urine of patients suffering from diabetic nephropathy and having impairment in the glomerulus in the course of progress of the nephropathy, and provides a diagnostic method for judging the condition of diabetic nephropathy by determination of the changes by use of the aforementioned quantitative determination method which permits simple and accurate determination of heparan sulfate contained in body fluid specimens; as well as a diagnostic kit for practicing the diagnostic method. Further, the inventors of the present invention have found that heparan sulfate changes quantitatively and/or qualitatively in blood of patients suffering from hepatic diseases and rheumatoid arthritis, and the present invention provides a diagnostic method for diagnosing hepatic diseases and rheumatoid arthritis by determination of the changes by use of the aforementioned quantitative determination method; as well as a diagnostic kit for practicing the diagnostic method.