COLD SHOCK PROTEIN GENE ISOLATED FROM STREPTOMYCES SP. AA8321 AND USES THEREOF

摘要

PURPOSE: A cold shock protein gene isolated from Streptomyces sp. AA8321 and uses thereof are provided, thereby increasing survival rate of useful microorganisms and improving low temperature tolerance of lactic acid bacteria or low temperature storage required useful microorganisms. CONSTITUTION: The cold shock protein gene csp isolated from Streptomyces sp. AA8321(KCTC 0518BP) has the nucleotide sequence set forth in SEQ ID NO:1. The cold shock protein isolated from Streptomyces sp. AA8321(KCTC 0518BP) has the amino acid sequence set forth in SEQ ID NO:2. The method for producing the cold shock protein comprises the steps of: constructing a recombinant expression vector containing the cold shock protein gene csp of SEQ ID NO:1; transforming E. coli with the recombinant expression vector containing the cold shock protein gene csp of SEQ ID NO:1 to produce E. coli LMG194(KCTC 10354BP); and culturing E. coli LMG194(KCTC 10354BP).