REPLICATION OF NUCLEIC ACID ARRAYS

摘要

A method of producing arrays of nucleic acids corresponding to a first set of nucleic acids immobilised on a first solid support (master array) includes furnishing on said first set of nucleic acids a second set of nucleic acids, each nucleic acid of the second set having at least a portion which is complementary to at least a portion of respective nucleic acid of the first set and being hybridised to it thereby. This may involve contact with a solution containing a mixture of nucleic acids, or synthesis using the first nucleic acids as primers and templates. The second set of nucleic acids are immobilised to a second solid support, either before or while they are hybridised to the first set of nucleic acids. Separation of the first and second solid supports provides said first support bearing the master array and said second support bearing a complementary array.