METHOD OF ANALYZING LIQUID SAMPLE AND ANALYTICAL APPARATUS

摘要

A method of analyzing a liquid sample with a solid substrate having fixed to the surface thereof two or more reactants which react with specific target substances at respective different reaction temperatures, wherein a temperature environment in which all the reactants can react with the respective specific target substances can be formed and a series of operations beginning with the reactions and ending with detection can be automated. In the method and apparatus, a liquid sample is analyzed by conducting: a step (a) in which the liquid sample present outside a reaction chamber is heated to a temperature higher than the reaction temperatures for the reactants; a step (b) in which the liquid sample present outside the reaction chamber is introduced into the reaction chamber; a step (c) in which the liquid sample present inside the reaction chamber is discharged from the reaction chamber; a step (d) in which the liquid sample present inside the reaction chamber and/or the liquid sample present outside the reaction chamber is cooled so that the temperature of the liquid sample declines through the reaction temperatures for all the reactants; and a step (e) in which the liquid sample cooled to each reaction temperature for the corresponding reactant is held in the reaction chamber.