发明名称 |
Epitope-tagged recombinant Growth Arrest Specific Gene 6 protein |
摘要 |
The present invention provides epitope-tagged mammalian recombinant Growth Arrest Specific Gene 6 (Gas6) proteins or polypeptides that retain their biological activity of Gas6, including a novel method of generating and purifying the aforesaid proteins or polypeptides. Such biolgically active eptitope-tagged Gas6 proteins or polypeptides can be used when the presence of Gas6 is required and/or when Gas6 antagonists and receptors need to be identified. Prior to being expressed from a mammalian cell, the recombinant Gas6 protein or polypeptide is tagged with either Flag or polyhistidine to the C-terminus. After expression, the protein or polypeptide is purified in a single step affinity purification using either anti-Flag antibody resin or nickle resin. The purified epitope-tagged mammalian recombinant Gas6 protein or polypeptide maintains its biological activity and can be used thereafter.
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申请公布号 |
US2004157774(A1) |
申请公布日期 |
2004.08.12 |
申请号 |
US20030671054 |
申请日期 |
2003.09.24 |
申请人 |
YANG JING;CHEN CAILIN;HU BING;ANDRADE-GORDON PATRICIA;JORDAN ROBERT |
发明人 |
YANG JING;CHEN CAILIN;HU BING;ANDRADE-GORDON PATRICIA;JORDAN ROBERT |
分类号 |
C07K14/47;(IPC1-7):A61K38/17;C07K14/705 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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