Immobilized cDNA libraries

摘要

A cDNA library in which sense strand cDNAs are immobilized at the 5'-side is provided. A known nucleotide sequence is artificially added to the 3'-side of an antisense strand cDNA (the first strand) and the 5'-side of the second strand is immobilized by using a primer complementary to the above nucleotide sequence. Thus, a cDNA library with excellent qualities, which contain the full-length cDNA at a high possibility, can be obtained.