| 摘要 |
PURPOSE: A composition for diagnosis of male infertility comprising primers detecting single nucleotide polymorphism(SNP) at 677th nucleotide of 5,10-methylenetetrahydrofolate reductase(MTHFR) gene is provided, thereby easily diagnosing male infertility, and preventing and treating male infertility because SNP at 677th nucleotide of MTHFR gene is a major reason for male infertility. CONSTITUTION: The composition for diagnosis of male infertility comprises a set of primers detecting single nucleotide polymorphism at 677th nucleotide of 5,10-methylenetetrahydrofolate reductase gene, consisting of a sense primer having the nucleotide sequences of SEQ ID NO: 1 or 3 and an antisense primer having the nucleotide sequences of SEQ ID NO: 2 or 4, and a sequence primer for pyrosequencing of PCR product, having the nucleotide sequence set forth in SEQ ID NO:5, wherein the male infertility is azoospermia or severe oligoasthenoteratozoospermia; and the SNP of MTHFR gene is selected from cytosine/cytosine(C/C), cytosine/thymine(C/T), and thymine/thymine(T/T).
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