| 摘要 |
<p>PURPOSE: An oligonucleotide for analyzing a target nucleotide sequence in a sample nucleic acid by hybridization and use thereof are provided, which oligonucleotide can be useful for analysis of a target nucleotide sequence, detecting the presence of the target nucleotide sequence, and identifying the nucleotide mutation of the target nucleotide sequence. CONSTITUTION: The oligonucleotide for analyzing a target nucleotide sequence in a sample nucleic acid by hybridization has the general structure of 5'-Xp-Xq-Zr-3' or 5'-Zr-Yq-Xp-3', wherein Xp represents a first hybridization portion having a specific hybridizing nucleotide sequence substantially complementary to the target nucleotide sequence in the sample nucleic acid to hybridize therewith; Yq represents a regulator portion comprising at least two universal bases or non-discriminatory base analogs; Zr represents a second hybridization portion having a pre-selected arbitrary nucleotide sequence; p, q and r represent the number of nucleotides; and X, Y and Z is deoxyribonucleotide or ribonucleotide.</p> |
