| 摘要 |
The invention relates to a method for the determination of an analyte in a sample by fluorescence correlation spectroscopy, the distance between the measurement volume in the sample and the optical excitation/detection direction being >=1 mm and the sample liquid being thermally insulated from the optical instrument. The method is especially suitable for the measurement of temperature-dependent processes, for example the determination of nucleic acid hybridization melting curves and/or for carrying out nucleic acid amplification reactions. A device suitable for carrying out the method according to the invention is furthermore disclosed.
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