Viscosity control during polynucleotide synthesis

摘要

A method of fabricating an array of biopolymer probes bound to a surface of a substrate. The method includes depositing drops, at least some of which contain probe precursors, onto the substrate surface so that the probe precursors bind to the surface through a linker. This can be repeated multiple times with the probe precursor deposited in a prior cycle becomes the linker for a probe precursor deposited in a subsequent cycle, so as to form the array. The deposited drops may have a viscosity modifier or the viscosities of them may be otherwise controlled as discussed herein. Kits and compositions are further provided.