METHOD OF SCREENING LIGHT CHAIN OF ANTIBDOY

摘要

A method of screening a common light chain which comprises: (a) the step of producing a host secreting the heavy chain of an antibody binding to a desired antigen; (b) the step of transferring an antibody light chain library into the host of the step (a) and thus producing libraries presenting antibodies consisting of the above heavy chain and the above light chain; (c) selecting a library presenting an antibody binding specifically to the desired antigen as described in the step (a); (d) the step of transferring the library selected in the step (c) into a host secreting the heavy chain of an antibody binding to a desired antigen, which is different from the antigen of the step (a), and thus producing libraries presenting antibodies consisting of the heavy chain and the light chain; and (e) selecting a library presenting an antibody binding specifically to the desired antigen as described in the step (d).