发明名称 |
PEROXIDE-DRIVEN CYTOCHROME P450 OXYGENASE VARIANTS |
摘要 |
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have a improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T. L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions. |
申请公布号 |
WO02083868(A3) |
申请公布日期 |
2004.08.05 |
申请号 |
WO2002US11954 |
申请日期 |
2002.04.16 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY;CIRINO, PATRICK, C.;ARNOLD, FRANCES, H. |
发明人 |
CIRINO, PATRICK, C.;ARNOLD, FRANCES, H. |
分类号 |
C07H21/04;C12N9/02;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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