A method to produce cloned embryos and adults from cultured cells particulary from embryonic stem (ES) cell lines

摘要

A method for cloning a non-human embryo comprises the steps of (a) collecting the nucleus of a cultured non-human embryonic stem or embryonic germ cell, (b) microinjecting the nucleus of (a) or at least a portion of thereof that includes the chromosomes into an enucleated oocytes to reconstitute the cell and (c) allowing the reconstituted cell to develop embryonically. Also described are methods for clonally deriving differentiated non-humans cells and modulating non- human embryonic development.