AFFINITY SCREENING USING ~ONE-BEAD-ONE-COMPOUND~ LIBRARIES

摘要

The invention provides putative ~drugable~ protein targets and actively binding ligands identified in an efficient and reproducible process by determining the affinity of protein mixtures to libraries of ligand compound s of defined size and composition. The libraries are used to isolate and identify previously unknown corresponding protein-ligand binding pairs from a mixture of proteins and a library of compounds, and are particularly useful to identify differentially selective protein-ligand binding pairs, for example, representing a single physiological state or several varied but related states, such as disease versus normal conditions. The invention also provide s processes for identifying such protein-ligand binding pairs.