发明名称 |
Process for producing cis-3-hydroxy-L-proline |
摘要 |
An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.
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申请公布号 |
US6767726(B2) |
申请公布日期 |
2004.07.27 |
申请号 |
US20020138632 |
申请日期 |
2002.05.06 |
申请人 |
KYOWA HAKKO KOGYO CO., LTD. |
发明人 |
OZAKI AKIO;MORI HIDEO;SHIBASAKI TAKESHI;ANDO KATSUHIKO;OCHIAI KEIKO;CHIBA SHIGERU;UOSAKI YOICHI |
分类号 |
C12N9/02;C12P13/24;(IPC1-7):C12N9/02;C12N1/20;C12N15/00;C07H21/04;C07K1/00 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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