| 摘要 |
The present invention relates to modified nucleotides and nucleosides and reagents to produce these. The modified nucleotides and nucleotides are assembled to larger oligonucleotides and oligonucleosides, which, for example, may be used for diagnostics of polymorphisms and for antisense therapy of various conditions. The oligonucleotides and oligonucleosides described in the invention have very good endonuclease resistance without compromising the RNA cleavage properties of RNase H wherein combinations of modifications with Y, Z, R or B are claimed: X=O or S, NH or NCH3, CH2 Or CH(CH3), Y=O, S, or NH or NCH3, CH2or CH(CH3); Z=O, S, or NH or NCH3, CH2 or CH(CH3); R=O or S, or NH or NCH3, CH2 or CH(CH3); B=A, C, G, T; 5-F/cl/BrU or -C, 6-thioguanine, 7-deazaguanine; alpha- or beta-D- (or L)ribo, xylo, arabino or lyxo configuration.
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