CELL GROWTH INHIBITOR USING SPHINGOSINE KINASE 2, METHOD OF CONSTRUCTING FUSED PROTEIN HAVING ITS NUCLEAR LOCALIZATION SIGNAL, METHOD OF SCREENING DRUG CANDIDATE, AND SCREENING KIT

摘要

It is intended to provide medically and industrially useful substances and methods based on the analysis of the function of sphingosine kinase 2 (SPHK2). As the results of the analysis on the function of sphingosine kinase 2 (SPHK2), it is found out that SPHK2 occurs mainly in the nucleus of a cell and has a nuclear localization signal (NLS) in its amino-terminal side, DNA synthesis in cells is inhibited by expressing SPHK2, SPHK2 should be present in the nucleus to exert its effect of inhibiting the DNA synthesis, etc. Therefore, SPHK2 is usable as a cell growth inhibitor and its NLS sequence is applicable to the construction of a novel fused protein or the like, which makes them medically and industrially useful.