| 摘要 |
PROBLEM TO BE SOLVED: To provide a method that can rapidly detect Eumycetes (true fungi) and can identify their genera or species. SOLUTION: Paying attention to that consensus sequences are observed in the genome of Eumycetes and the sequences specific to the genera or species of Eumycetes are noticed there, a primer comprising an oligonucleotide having a base sequence given by a specific sequence is used to detect the microorganism pathogenic of the human profound mycoses by using a nucleic acid amplification technique. Further, the amplified nucleic acids is subjected to the melting temperature analysis to carry out the identification and determination of the genera or species of the profound mycoses. In addition, the species of the fungi are identified by the system study of genes on the basis of the base sequence of the amplified products. COPYRIGHT: (C)2004,JPO&NCIPI
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