Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes

摘要

Methods for preparing cells that contain artificial chromosomes, methods for preparation of artificial chromosomes, methods for purification of artificial chromosomes, methods for targeted insertion of heterologous DNA into artificial chromosomes, and methods for delivery of the chromosomes to selected cells and tissues are provided. Also provided methods for producing transgenic plants and animals using the artificial chromosomes and the resulting transgenic organisms are provided.