METHOD OF DETECTING TARGET NUCLEIC ACID AND NUCLEIC ACID PROBE

摘要

The method for detecting a target nucleic acid includes the steps of: preparing a nucleic acid probe labeled with a donor fluorescent substance and an acceptor substance placed to allow occurrence of fluorescence energy transfer, the nucleic acid probe having a sequence complementary to at least part of the target nucleic acid; mixing a sample solution with the nucleic acid probe to hybridize the target nucleic acid and the nucleic acid probe; and after the step of mixing, allowing the sample solution to act with sequence-independent nuclease and detecting the target nucleic acid from a change in the fluorescence intensity of the sample solution. This enables both enhancement of the degree of freedom in the design of the nucleic acid probe and simplification of the operation. <IMAGE>