发明名称 |
METHOD AND DEVICE FOR OPTIMIZING A NUCLEOTIDE SEQUENCE FOR THE PURPOSE OF EXPRESSION OF A PROTEIN |
摘要 |
The invention relates to a method for optimizing a nucleotide sequence for the purpose of expression of a protein on the basis of the amino acid sequence of said protein. According to the inventive method, a test sequence with m optimization positions is determined for a defined region, in which positions the codon usage is varied. The optimum codon usage on said optimization positions is determined by means of a power function and one or more codons of said optimum usage are determined as the codons of the optimized nucleotide sequence. These steps are iterated, while the codons of the optimized nucleotide sequence determined in the preceding steps remain unchanged during the subsequent iteration steps. The invention further relates to a device for carrying out said method. |
申请公布号 |
WO2004059556(A2) |
申请公布日期 |
2004.07.15 |
申请号 |
WO2003EP14850 |
申请日期 |
2003.12.23 |
申请人 |
GENEART GMBH;RAAB, DAVID;GRAF, MARCUS;NOTKA, FRANK;WAGNER, RALF |
发明人 |
RAAB, DAVID;GRAF, MARCUS;NOTKA, FRANK;WAGNER, RALF |
分类号 |
C07H21/00;C12M1/36;C12N15/11;C12N15/12;C12N15/19;C12N15/24;C12N15/27;C12N15/29;C12N15/31;C12N15/52;C12P21/00;G06F19/18;G06F19/22 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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