摘要 |
A post-translationally processed hedgehog protein mutant which exhibits a molecular weight of 22±1 kDa under alkylating conditions, exhibits a molecular weight of 24±1 kD under reducing conditions, is stabilized with respect to its activity by suramin is inactivated when 8 or more amino acids are cleaved N-terminally is inactivated by 90% or more when incubated with 10 mmol/l DTE for 2.5 hours at 37° C., induces an activity for alkaline phosphatase of ca. 90 nmol pNP/min/mg at a concentration of 5 nmol/l in the presence of suramin, is not modified by cholesterol, exhibits an activity that is increased many times.
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