IMPROVED LUCIFERASE-BASED ASSAYS

摘要

A method and kit is provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferas e enzyme activity from interference of the compound and minize interference by at least about 10% relative to an assay not having tolerance enhancement age nt.