HIGH THROUGHPUT AUTOMATIC NUCLEIC ACID ISOLATION AND QUANTITATION METHODS

摘要

A high throughput RNA laboratory protocol is provided for the extraction and maintenance of a sufficient quantity of high quality RNA during sample preparation in order to analyze several genes at a time with assistance of computer analysis. The subject invention includes a method for analyzing RNA comprising the steps of extracting RNA from a complex biological construct in sufficient quantities to provide accurate RNA data, transferring RNA to an apparatus that maintains the RNA and necessary reagents at a temperature of between about 0 to 10° C, and analyzing RNA with a computer generated mathematical analysis of the data to access the presence of RNA and ultimately test the efficacy of a drug.