摘要 |
The present invention deals with the leading sequence of human source gene, gene vector and gene expression strategies. The invention include the following: (1) Using DNA sequence without pivot physiological function-related gene in short arms of human group D, G chromosomes, or a DNA sequence sharing 50% or over 50% identity to the sequence select from human D, G group chromosomes as leading sequence for gene targeting; (2) Construct the gene vector containing the leading sequence above; (3) Nucleolus organizing region in D, G group chromosomes mentioned in the procedure(1) is used as the target site, the gene of interest is integrated into the short arms where the gene expresses actively in D, G group chromosomes of human cells. The present invention provides a novel gene leading sequence by which the gene vector construction and gene expression strategies are obtained. The gene expression strategies can be used for human gene therapy and manufacturing protein.
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