| 摘要 |
Described is a novel purification tag, SNUT, based on the gene product of a sortase gene, in particular the srtA gene of Staphylococcus aureus and uses thereof. Also provided are expression constructs comprising DNA enoding the tag for the production of recombinant polypeptides. The tag can be used in methods of purifying soluble domains of a number of proteins previously not able to be isolated efficiently and methods of inducing and/or enhancing an immune response to an antigen of interest. |
