REAL-TIME PCR PRIMERS AND PROBES FOR IDENTIFICATION AS RALSTONIA SOLANACEARUM RACE 3, BIOVAR 2 IN POTATO

摘要

Ralstonia solanacearum, the casual agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assays technique for detecting potato tubers latently infected with R. solanacearum. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (preenrichment on agar or in liquid medium) assays but not by direct real-time PCR.