发明名称 |
Beta-catenin oligonucleotide michrochip and method for detecting beta-catenin mutations employing same |
摘要 |
<p>A beta -catenin oligonucleotide microchip for detecting beta -catenin mutations comprises oligonucleotides fixed on the surface of a solid matrix. The oligonucleotides are designed to detect a variety of mutations at mutational hot spots of the beta -catenin gene. Independent claims are also included for: (1) manufacture of the beta -catenin oligonucleotide microchip comprising: (a) mixing each oligonucleotide in a micro spotting solution and distributing to a well plate; (b) spotting the oligonucleotides on the surface of a solid matrix using a microarrayer; (c) fixing the oligonucleotides on the solid matrix surface and washing; (d) denaturing the fixed oligonucleotides by soaking the solid matrix in 95 [deg]C water and then treating the solid matrix with a sodium borohydride solution; and (e) washing and drying the solid matrix; and (2) detecting a beta -catenin mutation using the microchip, comprising: (a) preparing a fluorescent dye-labeled DNA sample from the blood of a subject; (b) reacting the labeled DNA sample with oligonucleotide spots on the beta-catenin oligo chip; (c) washing the reacted oligo chip to remove unbound sample DNA; (d) detecting the mode of hybridization of specific oligonucleotide spots using a fluorescence reader; and (e) examining the presence of gene mutation.</p> |
申请公布号 |
EP1437417(A1) |
申请公布日期 |
2004.07.14 |
申请号 |
EP20030019265 |
申请日期 |
2003.08.26 |
申请人 |
NATIONAL CANCER CENTER |
发明人 |
PARK, JAE-GAHB;KIM, IL-JIN;KANG, HIO-CHUNG;PARK, JAE-HYUN |
分类号 |
G01N33/53;C12M1/00;C12M1/34;C12N15/09;C12Q1/68;G01N33/574;G01N33/68;G01N37/00;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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