METHOD OF PREPARING BASEMENT MEMBRANE

摘要

Preparing a basement membrane, comprising culturing cells on a sugar chain-provided substrate, where the basal face of cells capable of forming a basement membrane has been coated with a polymer having a sugar chain capable of localizing a receptor with an effect of accumulating basement membrane-constituting components, is new. Independent claims are also included for: (1) a tissue model made from the from the basement membrane prepared by the novel method; (2) testing tissue kit containing the tissue model; (3) constructing a basement membrane specimen by treating cells that are capable of forming a basement membrane and have been adhered to a support via the basement membrane with use of a solvent and an alkaline solution to solubilize and remove lipid components of the cells; (4) basement membrane specimen thus constructed; (5) reconstituting tissues by disseminating and culturing the already-specified cells on the basement membrane specimen or an amorphous precipitate specimen of the basement membrane-constituting components for culturing; (6) reconstituted artificial tissues thus produced; (7) testing safety or toxicity of a sample by using the reconstituted tissues of (6); (8) basement membrane specimens or artificial tissues formed on a protein support is tentatively adhered to a plastic surface via a hydrophobic bond adsorption polymer having a linear carbon skeleton with a hydrophobic nature and a functional group capable of reacting with a protein in the molecule; and (9) producing basement membrane specimens or artificial tissues for maintaining on the structure of a basement membrane or enabling transplantation by producing the materials so that the protein support carrying them on can be physically separated from the plastic surface when needed.