| 摘要 |
<p>The invention relates to novel vectors, to DNA vaccines and gene therapeutics containing said vectors, to methods for the preparation of the vectors and DNA vaccines and gene therapeutics containing the vectors, and to therapeutic uses of said vectors. Specifically, the present invention relates to novel vectors comprising (a) an expression cassette of a gene of a nuclear-anchoring protein, which contains (i) a DNA binding domain capable of binding to a specific DNA sequence and (ii) a functional domain capable of binding to a nuclear component, (b) a multimerized DNA forming a binding site for the anchoring protein of a nuclear-anchoring protein, and (c) one or more expression cassettes of a DNA sequence encoding a growth factor of the vascular endothelial growth factor (VEGF) family, a receptor of the vascular endothelial growth factor receptor (VEGFR) family, a growth factor of the angiopoetin family, a member of an ephrin family or a member of an Eph-receptor family. In particular the invention relates to vectors that lack a papilloma virus origin of replication and an origin of replication functional in a mammalian cell.</p> |
