MEANS AND METHODS FOR PRODUCING A PROTEIN THROUGH CHROMATIN OPENERS THAT ARE CAPABLE OF RENDERING CHROMATIN MORE ACCESSIBLE TO TRANSCRIPTION FACTORS

摘要

The invention is concerned with means and methods for providing a cell with a protein expression unit comprising providing a nucleic acid comprising said unit with a nucleic acid encoding a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener), wherein said opener is present in said cell. Preferred openers comprise histone modification proteins, chromatin remodeling proteins and trithorax group proteins or equivalents. The cells thus generated and nucleic acids encoding such openers are provided. Openers are preferred in the context of STAR and TRAP sequences.