METHOD AND DEVICE FOR PCR-AMPLIFICATION AND DETECTION OF NUCLEOTIDE SEQUENCES

摘要

The invention relates to a DNA-Chip comprising a flat carrier and an array o f spots containing probe molecules (oligonucleotides) which are arranged on sa id carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the probe molecules and target molecules (DNA fragments) applied by means of an analyte solution. In order to increase the sensitivity or the binding specif ic measuring effects of the biochip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing probe molecule s and which is permeable to target molecules.