IMMUNOCAPTURE AND FUNCTIONAL ASSAY FOR MITOCHONDRIAL F1/F0 ATPASE

摘要

Fl/F0 ATPase defects are one of the causes of diseases and disorders associated with mitochondrial respiratory chain disorders, including genetically related diseases and late onset neurodegenerative diseases. The invention provides methods for using an anti ­F1/F0 ATPase capture antibody and antibodies that react with subunits of F1 /F0 ATPase to aid in diagnosis of F1/F0 ATPase deficiencies and that can be used for rapid screening to detect onset or stage of a variety of diseases. The invention further provides immunological methods for screening agents, such as drugs, for their effect upon F1/F0 ATPase activity and for screening patients to detect those with impairment of F1/F0 ATPase activity and those suspected of having a late onset disease associated with post­translational modification of one or more subunits of F1/F0 ATPase.