发明名称 |
D-AMINO ACID OXIDASE HAVING EXCELLENT HEAT-STABILITY, ITS GENE, RECOMBINANT DNA AND METHOD FOR PRODUCING D-AMINO ACID OXIDASE HAVING EXCELLENT HEAT-STABILITY |
摘要 |
<P>PROBLEM TO BE SOLVED: To provide a D-amino acid oxidase having excellent heat-stability, its gene, a recombinant DNA and a method for producing the D-amino acid oxidase having excellent heat-stability. <P>SOLUTION: This invention relates to (1) the D-amino acid oxidase having an amino acid sequence obtained by replacing the 42nd amino acid with cysteine or the 246th amino acid with tyrosine in a specific amino acid sequence; (2) the D-amino acid oxidase gene encoding the amino acid sequence described above wherein the 42nd amino acid is replaced with cysteine or the 246th amino acid is replaced with tyrosine; (3) the recombinant DNA produced by inserting the D-amino acid oxidase gene described in the item (2) into a vector DNA; (4) a transformant or a transductant; and (5) the method for producing the D-amino acid oxidase having excellent heat-stability. The D-amino acid oxidase having excellent heat-stability is produced by this invention and the enzyme is useful in an industrial field by advantageously using it as a diagnostic enzyme, or the like, in the form of a determination kit. <P>COPYRIGHT: (C)2004,JPO&NCIPI |
申请公布号 |
JP2004180624(A) |
申请公布日期 |
2004.07.02 |
申请号 |
JP20020353693 |
申请日期 |
2002.12.05 |
申请人 |
KIKKOMAN CORP |
发明人 |
JOKA MIKIO;KAJIYAMA NAOKI |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/06 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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