| 摘要 |
A method for optimizing the function of a sensor device, wherein a protein, such as an enzyme, is immobilized in a matrix, the matrix is adhered to the device and the protein comprises at least one reactive moiety. The immobilized protein is reacted with a redox agent, wherein the reacting increases the stability of the immobilized protein. The device can be sterilized after the reacting step. A typical device comprises a glucose sensor in which glucose oxidase is embedded in a polymer matrix adhered to the device, the reactive moiety comprises FADH2, and the redox agent is the reducing agent, sodium borohydride. Also provided is a method of measuring an analyte in a tissue of a subject comprising introducing a sensor device of the invention into the tissue of the subject and detecting the signal generated by the protein. The amount of signal corresponds to the amount of analyte.
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