Method for detecting transcription templates

摘要

Methods are provided for detecting the sense and antisense transcripts and for determining template strand of a genomic DNA. Exemplary methods include reverse transcribing transcripts without second strand synthesis. The resulting single stranded stranded DNA is labeled and detected using nucleic acid probe arrays. In a particularly preferred embodiment, actinomycin is used to inhibit the synthesis of second strand cDNA during reverse transcription.