Anti-Kazlauskas lipases

摘要

Nucleic acid (A) that encodes a polypeptide (B) with the biological activity of an anti-Kazlauskas lipase is new. (B) is a 294 amino acid (aa) sequence (SEQ ID: 2). Independent claims are also included for the following: (1) DNA construct comprising (A) and a heterologous promoter; (2) vector comprising (A) or the construct of (1); (3) host cell containing (A), the construct of (1) or the vector of (2); (4) methods for preparing (A); (5) (B) as new compounds; (6) methods for preparing (B); (7) method for preparing aralkyl esters of formula (I) by reacting a stereoisomeric mixture of the corresponding alcohol (III) with ester (IV) in presence of (B); and (8) method for preparing aralkanol (IIa) by hydrolysis of a stereoisomeric mixture of esters (Ia) in presence of (B). Ar = 5-14C aryl; R = cyano, 1-12C (halo)alkyl, 5-11C aralkyl or a group A-CO-D, A-D, A-SO2R3, A-SO3W, A-COW or A-N3; A = 1-8C alkylene or is absent; D = R2, OR2, NHR3 or N(R3)2; R2 = 1-8C (halo)alkyl, 6-15C aralkyl or 5-14C aryl; each R3 = 1-8C alkyl, 6-15C aralkyl or 6-14C aryl, or both together complete cyclic amino; W = hydroxy, amino or OM; M = (equivalent of) alkali metal, alkaline earth metal or (organic) ammonium ion; R4 = 1-12C alkyl, 4-10C aryl, 5-11C aryl-alkyl, 2-8C alkenyl or 1-12C haloalkyl; and R1 = 1-12C (halo)alkyl, 5-11C aralkyl or 4-10C aryl.