METHOD FOR PRODUCING HUMAN INTERLEUKIN-11 USING YEAST

摘要

PURPOSE: A method for producing human interleukin-11 using yeast is provided, thereby effectively producing human interleukin-11 from yeast in higher yield. CONSTITUTION: The method for producing human interleukin-11 using yeast comprises the steps of: preparing recombinant cDNA encoding human interleukin-11; constructing an expression vector pYLBC A/G UB rIL-11 containing ubiquitin gene, alanine codon and human interleukin-11 cDNA; transforming Saccharomyces cerevisiae DC04 with the expression vector pYLBC A/G UB rIL-11 to produce Saccharomyces cerevisiae DC04/pYLBC A/G UB rIL-11(KCTC 8721P); and culturing Saccharomyces cerevisiae DC04/pYLBC A/G UB rIL-11(KCTC 8721P) and recovering from the cultured medium.