发明名称 |
METHOD OF AFFINITY CROSS-LINKING BIOLOGICALLY ACTIVE, IMMUNOGENIC PEPTIDES TO ANTIBODIES |
摘要 |
A method of affinity cross-linking a peptide to an antibody by photo-chemically activating an azido compound in a peptide including said azido compound; adding an antibody to the photochemically activated peptide; and allowing the photochemically activated peptide and the antibody to react. The azido compound has an affinity for a hydrophobic structure in the variable domain of the antibody which binds to nucleotides or nucleosides, binding the peptide into a native binding pocket of the immunoglobulin (Ig) structure of an antibody. The site of cross-linking is located away from the antigen binding site in the Fv domain avoiding the compromise of antigen recognition. A composition of a peptide cross-linked to an antibody is also disclosed. |
申请公布号 |
EP1017725(A4) |
申请公布日期 |
2004.06.30 |
申请号 |
EP19980949410 |
申请日期 |
1998.09.18 |
申请人 |
INNEXUS BIOTECHNOLOGY INTERNATIONAL LTD |
发明人 |
KOHLER, HEINZ |
分类号 |
A61K39/00;C07K14/47;C07K14/77;C07K16/00;C07K16/42 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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